Review



ecis instrument model zθ applied biophysics  (Applied BioPhysics)


Bioz Verified Symbol Applied BioPhysics is a verified supplier
Bioz Manufacturer Symbol Applied BioPhysics manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Applied BioPhysics ecis instrument model zθ applied biophysics
    Effect of aging on pulmonary microvascular endothelial cell (PMVEC) permeability under basal conditions. A) Monolayer resistance was assessed in PMVEC from young and aged mice by electric cell-substrate impedance sensing <t>(ECIS).</t> Compared to young, PMVEC from aged mice exhibited significantly decreased monolayer resistance 26 hours after seeding. B) Immunofluorescent (IF) staining of vascular endothelial (VE)-cadherin (RED) and local leak of NeutrAvidin (GREEN) was carried out in PMVEC monolayers from young and aged animals. PMVEC from young mice exhibited continuous VE-cadherin IF staining around the periphery of the cells, and this was associated with minimal leak. In contrast, PMVEC from aged mice exhibited discontinuous VE-cadherin IF staining (white arrows) and significantly increased avidin leak, with areas of leak colocalized directly at paracellular regions of VE-cadherin discontinuity. For ECIS experiments, n = 4; *p<0.05; Repeated measures two-way ANOVA. For NeutrAvidin Leak experiments, n = 3; ***p<0.001; Unpaired t-test. Scale bar = 100 μm.
    Ecis Instrument Model Zθ Applied Biophysics, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 637 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecis+model+z%CE%B8+instrument/ECIS+Z+Theta/bio_rxiv__2025__07__29__667420-58-20-24
    Average 96 stars, based on 637 article reviews
    ecis instrument model zθ applied biophysics - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "The role of aging on endothelial cell-cell junctions and pulmonary microvascular permeability"

    Article Title: The role of aging on endothelial cell-cell junctions and pulmonary microvascular permeability

    Journal: bioRxiv

    doi: 10.1101/2025.07.29.667420

    Effect of aging on pulmonary microvascular endothelial cell (PMVEC) permeability under basal conditions. A) Monolayer resistance was assessed in PMVEC from young and aged mice by electric cell-substrate impedance sensing (ECIS). Compared to young, PMVEC from aged mice exhibited significantly decreased monolayer resistance 26 hours after seeding. B) Immunofluorescent (IF) staining of vascular endothelial (VE)-cadherin (RED) and local leak of NeutrAvidin (GREEN) was carried out in PMVEC monolayers from young and aged animals. PMVEC from young mice exhibited continuous VE-cadherin IF staining around the periphery of the cells, and this was associated with minimal leak. In contrast, PMVEC from aged mice exhibited discontinuous VE-cadherin IF staining (white arrows) and significantly increased avidin leak, with areas of leak colocalized directly at paracellular regions of VE-cadherin discontinuity. For ECIS experiments, n = 4; *p<0.05; Repeated measures two-way ANOVA. For NeutrAvidin Leak experiments, n = 3; ***p<0.001; Unpaired t-test. Scale bar = 100 μm.
    Figure Legend Snippet: Effect of aging on pulmonary microvascular endothelial cell (PMVEC) permeability under basal conditions. A) Monolayer resistance was assessed in PMVEC from young and aged mice by electric cell-substrate impedance sensing (ECIS). Compared to young, PMVEC from aged mice exhibited significantly decreased monolayer resistance 26 hours after seeding. B) Immunofluorescent (IF) staining of vascular endothelial (VE)-cadherin (RED) and local leak of NeutrAvidin (GREEN) was carried out in PMVEC monolayers from young and aged animals. PMVEC from young mice exhibited continuous VE-cadherin IF staining around the periphery of the cells, and this was associated with minimal leak. In contrast, PMVEC from aged mice exhibited discontinuous VE-cadherin IF staining (white arrows) and significantly increased avidin leak, with areas of leak colocalized directly at paracellular regions of VE-cadherin discontinuity. For ECIS experiments, n = 4; *p<0.05; Repeated measures two-way ANOVA. For NeutrAvidin Leak experiments, n = 3; ***p<0.001; Unpaired t-test. Scale bar = 100 μm.

    Techniques Used: Permeability, Electric Cell-substrate Impedance Sensing, Staining, Avidin-Biotin Assay

    Related Articles

    Electric Cell-substrate Impedance Sensing:

    Article Title: Inactivation of Cancer-Associated-Fibroblasts Disrupts Oncogenic Signaling in Pancreatic Cancer Cells and Promotes Its Regression
    Article Snippet: .. Impedance (Z), capacitance (C) and resistance (R) were monitored for 60 hours by an ECIS Model ZΘ instrument (Applied BioPhysics Inc., Troy, NY) and normalized to Z0. ..

    Article Title: ER stress sensor, glucose regulatory protein 78 (GRP78) regulates redox status in pancreatic cancer thereby maintaining “stemness”
    Article Snippet: .. Impedance (Z), capacitance (C), and resistance (R) were monitored for 48 h by an ECIS Model ZΘ instrument (Applied BioPhysics Inc.) and normalized to Z0. ..

    Article Title: Inactivation of cancer-associated-fibroblasts (CAF) disrupts oncogenic signaling in pancreatic cancer cells and promotes its regression
    Article Snippet: .. Impedance (Z), capacitance (C) and resistance (R) were monitored for 60 hours by an ECIS Model ZΘ instrument (Applied BioPhysics Inc., Troy, NY) and normalized to Z 0 . ..



    Similar Products

    ecis instrument model zθ applied biophysics  (Applied BioPhysics)
    96
    Applied BioPhysics ecis instrument model zθ applied biophysics
    Effect of aging on pulmonary microvascular endothelial cell (PMVEC) permeability under basal conditions. A) Monolayer resistance was assessed in PMVEC from young and aged mice by electric cell-substrate impedance sensing <t>(ECIS).</t> Compared to young, PMVEC from aged mice exhibited significantly decreased monolayer resistance 26 hours after seeding. B) Immunofluorescent (IF) staining of vascular endothelial (VE)-cadherin (RED) and local leak of NeutrAvidin (GREEN) was carried out in PMVEC monolayers from young and aged animals. PMVEC from young mice exhibited continuous VE-cadherin IF staining around the periphery of the cells, and this was associated with minimal leak. In contrast, PMVEC from aged mice exhibited discontinuous VE-cadherin IF staining (white arrows) and significantly increased avidin leak, with areas of leak colocalized directly at paracellular regions of VE-cadherin discontinuity. For ECIS experiments, n = 4; *p<0.05; Repeated measures two-way ANOVA. For NeutrAvidin Leak experiments, n = 3; ***p<0.001; Unpaired t-test. Scale bar = 100 μm.
    Ecis Instrument Model Zθ Applied Biophysics, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecis+model+z%CE%B8+instrument/ECIS+Z+Theta/bio_rxiv__2025__07__29__667420-58-20-24
    Average 96 stars, based on 1 article reviews
    ecis instrument model zθ applied biophysics - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    ecis model zθ instrument  (Applied BioPhysics)
    90
    Applied BioPhysics ecis model zθ instrument
    Effect of aging on pulmonary microvascular endothelial cell (PMVEC) permeability under basal conditions. A) Monolayer resistance was assessed in PMVEC from young and aged mice by electric cell-substrate impedance sensing <t>(ECIS).</t> Compared to young, PMVEC from aged mice exhibited significantly decreased monolayer resistance 26 hours after seeding. B) Immunofluorescent (IF) staining of vascular endothelial (VE)-cadherin (RED) and local leak of NeutrAvidin (GREEN) was carried out in PMVEC monolayers from young and aged animals. PMVEC from young mice exhibited continuous VE-cadherin IF staining around the periphery of the cells, and this was associated with minimal leak. In contrast, PMVEC from aged mice exhibited discontinuous VE-cadherin IF staining (white arrows) and significantly increased avidin leak, with areas of leak colocalized directly at paracellular regions of VE-cadherin discontinuity. For ECIS experiments, n = 4; *p<0.05; Repeated measures two-way ANOVA. For NeutrAvidin Leak experiments, n = 3; ***p<0.001; Unpaired t-test. Scale bar = 100 μm.
    Ecis Model Zθ Instrument, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecis+model+z%CE%B8+instrument/ecis+z%CE%B8+instrument/pmc06372649-222-14-18
    Average 90 stars, based on 1 article reviews
    ecis model zθ instrument - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    ecis model zθ instrument with a 96-well array station  (Applied BioPhysics)
    90
    Applied BioPhysics ecis model zθ instrument with a 96-well array station
    Effect of aging on pulmonary microvascular endothelial cell (PMVEC) permeability under basal conditions. A) Monolayer resistance was assessed in PMVEC from young and aged mice by electric cell-substrate impedance sensing <t>(ECIS).</t> Compared to young, PMVEC from aged mice exhibited significantly decreased monolayer resistance 26 hours after seeding. B) Immunofluorescent (IF) staining of vascular endothelial (VE)-cadherin (RED) and local leak of NeutrAvidin (GREEN) was carried out in PMVEC monolayers from young and aged animals. PMVEC from young mice exhibited continuous VE-cadherin IF staining around the periphery of the cells, and this was associated with minimal leak. In contrast, PMVEC from aged mice exhibited discontinuous VE-cadherin IF staining (white arrows) and significantly increased avidin leak, with areas of leak colocalized directly at paracellular regions of VE-cadherin discontinuity. For ECIS experiments, n = 4; *p<0.05; Repeated measures two-way ANOVA. For NeutrAvidin Leak experiments, n = 3; ***p<0.001; Unpaired t-test. Scale bar = 100 μm.
    Ecis Model Zθ Instrument With A 96 Well Array Station, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecis+model+z%CE%B8+instrument/ecis+z%CE%B8+96+well+array+station/pmc05381265-141-27-30
    Average 90 stars, based on 1 article reviews
    ecis model zθ instrument with a 96-well array station - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    electronic cell–substrate impedance sensing (ecis) instrument model zθ  (Applied BioPhysics)
    90
    Applied BioPhysics electronic cell–substrate impedance sensing (ecis) instrument model zθ
    Effect of aging on pulmonary microvascular endothelial cell (PMVEC) permeability under basal conditions. A) Monolayer resistance was assessed in PMVEC from young and aged mice by electric cell-substrate impedance sensing <t>(ECIS).</t> Compared to young, PMVEC from aged mice exhibited significantly decreased monolayer resistance 26 hours after seeding. B) Immunofluorescent (IF) staining of vascular endothelial (VE)-cadherin (RED) and local leak of NeutrAvidin (GREEN) was carried out in PMVEC monolayers from young and aged animals. PMVEC from young mice exhibited continuous VE-cadherin IF staining around the periphery of the cells, and this was associated with minimal leak. In contrast, PMVEC from aged mice exhibited discontinuous VE-cadherin IF staining (white arrows) and significantly increased avidin leak, with areas of leak colocalized directly at paracellular regions of VE-cadherin discontinuity. For ECIS experiments, n = 4; *p<0.05; Repeated measures two-way ANOVA. For NeutrAvidin Leak experiments, n = 3; ***p<0.001; Unpaired t-test. Scale bar = 100 μm.
    Electronic Cell–Substrate Impedance Sensing (Ecis) Instrument Model Zθ, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecis+model+z%CE%B8+instrument/electrical+cell+substrate+impedance+sensing+system+ecis/10__1177_slash_0883911516688482-74-8-16
    Average 90 stars, based on 1 article reviews
    electronic cell–substrate impedance sensing (ecis) instrument model zθ - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of aging on pulmonary microvascular endothelial cell (PMVEC) permeability under basal conditions. A) Monolayer resistance was assessed in PMVEC from young and aged mice by electric cell-substrate impedance sensing (ECIS). Compared to young, PMVEC from aged mice exhibited significantly decreased monolayer resistance 26 hours after seeding. B) Immunofluorescent (IF) staining of vascular endothelial (VE)-cadherin (RED) and local leak of NeutrAvidin (GREEN) was carried out in PMVEC monolayers from young and aged animals. PMVEC from young mice exhibited continuous VE-cadherin IF staining around the periphery of the cells, and this was associated with minimal leak. In contrast, PMVEC from aged mice exhibited discontinuous VE-cadherin IF staining (white arrows) and significantly increased avidin leak, with areas of leak colocalized directly at paracellular regions of VE-cadherin discontinuity. For ECIS experiments, n = 4; *p<0.05; Repeated measures two-way ANOVA. For NeutrAvidin Leak experiments, n = 3; ***p<0.001; Unpaired t-test. Scale bar = 100 μm.

    Journal: bioRxiv

    Article Title: The role of aging on endothelial cell-cell junctions and pulmonary microvascular permeability

    doi: 10.1101/2025.07.29.667420

    Figure Lengend Snippet: Effect of aging on pulmonary microvascular endothelial cell (PMVEC) permeability under basal conditions. A) Monolayer resistance was assessed in PMVEC from young and aged mice by electric cell-substrate impedance sensing (ECIS). Compared to young, PMVEC from aged mice exhibited significantly decreased monolayer resistance 26 hours after seeding. B) Immunofluorescent (IF) staining of vascular endothelial (VE)-cadherin (RED) and local leak of NeutrAvidin (GREEN) was carried out in PMVEC monolayers from young and aged animals. PMVEC from young mice exhibited continuous VE-cadherin IF staining around the periphery of the cells, and this was associated with minimal leak. In contrast, PMVEC from aged mice exhibited discontinuous VE-cadherin IF staining (white arrows) and significantly increased avidin leak, with areas of leak colocalized directly at paracellular regions of VE-cadherin discontinuity. For ECIS experiments, n = 4; *p<0.05; Repeated measures two-way ANOVA. For NeutrAvidin Leak experiments, n = 3; ***p<0.001; Unpaired t-test. Scale bar = 100 μm.

    Article Snippet: Cells were grown in complete DMEM, and resistance was continuously monitored at 4000 Hz with an electric cell-substrate impedance sensing (ECIS) instrument (Model Zθ, Applied Biophysics).

    Techniques: Permeability, Electric Cell-substrate Impedance Sensing, Staining, Avidin-Biotin Assay